Behavior of Primary Human Oral Keratinocytes Grown on Invisalign® SmartTrack® Material

Orthodontic clear aligner treatment is gaining tremendous popularity. The world market leader Align Technology® and its product Invisalign®. Although numerous patients are treated with Invisalign® aligners, only little known about the cellular effects of material on oral epithelial cells. In present study, we aimed to investigate SmartTrack® directly cultured primary human keratinocytes (HOKs). Cell morphology behavior were investigated by scanning electron microscopy bright field microscopy. Aligner viability detected cell-counting-kit (CCK)-8 live/dead staining. Gene expression several inflammatory barrier proteins was assessed qPCR. Cells tissue culture plastic served as control. proliferation/viability significantly lower in cells (p < 0.05) comparison Live/dead staining did not reveal an increase number dead surfaces. After two seven days incubation, interleukin (IL)-6 decreased, IL-8 increased HOKs intercellular adhesion molecule 1 (ICAM-1) decreased after days. markers showed that integrin (ITG)-α6 A significant decrease ITG-β4 E-cadherin levels compared control could be seen We find any cytotoxic effect, but alterations cell’s functions reaction obvious. Clinical studies required give further insights into clinical reactions underlying this quickly expanding orthodontic appliance.